Phenolic and flavonoid content and in vitro inhibitory effect of some Amazonian fruit juices on CYP3A4 activity

ABSTRACT Many foods interact with drugs and may cause changes in the pharmacological effects of co-administered therapeutic agent. The enzyme CYP3A4, which belongs to cytochrome P450 complex, is responsible for metabolism most currently on market involved many drug interactions. Hence, interaction this juices some fruits, such as grapefruit, can affect pharmacokinetics various drugs. However, native fruits from Amazon region have not yet been target type research. We determined total polyphenols flavonoids Amazonian açaí (Euterpe precatoria), buriti (Mauritia flexuosa), camu-camu (Myrciaria dubia), cubiu (Solanum sessiliflorum), cupuaçu (Theobroma grandiflorum), jenipapo (Genipa americana), taperebá (Spondias mombin) evaluated each fruit juice CYP3A4 activity, using star (Averrhoa carambola) positive control. Açaí presented highest content (102.6 ± 7.2 µg gallic acid equivalent (GAE) per mL 0.6 quercetin (QE) mL, respectively). All were able inhibit activity CYP3A4. There was no residual drug-metabolizing açai, buriti, cubiu, camu-camu, juice, while cupuaçu, control, 44.3, 54.3 20.2%, respectively. Additional studies should identify phytocompound(s) inhibition clarify mechanisms phenomenon.